Labeling:Article Title: Super-resolution microscopy reveals focal organization of ER-associated Y-complexes in mitosis.
Article Snippet: .. In brief, Nup96-SNAP was labeled with the BG-AF647 dye (New England Biolabs, S9136S) 10 of 13 EMBO reports 24: e56766 | 2023 2023 The Authors D ow nloaded from https://w w w .em bopress.org on January 25, 2024 from IP 220.158.144.59. at a concentration of 1 lM in 0.5% BSA in PBS at RT for 2 h. The cells were then incubated with primary antibodies (phospho-H3, Climp63) for 1 h. Subsequently, cells were washed with PBS and then incubated with secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 546, Thermo Fisher Scientific, A11034, A11003) at RT for 30 min and washed with PBS. .. After sample preparation, the smart kit imaging buffer (Abbelight) was added to the cells prior to SMLM imaging.
Article Title: Super‐resolution microscopy reveals focal organization of ER ‐associated Y‐complexes in mitosis
Article Snippet: .. In brief, Nup96‐SNAP was labeled with the BG‐AF647 dye (New England Biolabs, S9136S) at a concentration of 1 μM in 0.5% BSA in PBS at RT for 2 h. The cells were then incubated with primary antibodies (phospho‐H3, Climp63) for 1 h. Subsequently, cells were washed with PBS and then incubated with secondary antibodies (goat anti‐rabbit Alexa Fluor 488, goat anti‐mouse Alexa Fluor 546, Thermo Fisher Scientific, A11034, A11003) at RT for 30 min and washed with PBS. .. After sample preparation, the smart kit imaging buffer (Abbelight) was added to the cells prior to SMLM imaging.
Concentration Assay:Article Title: Super-resolution microscopy reveals focal organization of ER-associated Y-complexes in mitosis.
Article Snippet: .. In brief, Nup96-SNAP was labeled with the BG-AF647 dye (New England Biolabs, S9136S) 10 of 13 EMBO reports 24: e56766 | 2023 2023 The Authors D ow nloaded from https://w w w .em bopress.org on January 25, 2024 from IP 220.158.144.59. at a concentration of 1 lM in 0.5% BSA in PBS at RT for 2 h. The cells were then incubated with primary antibodies (phospho-H3, Climp63) for 1 h. Subsequently, cells were washed with PBS and then incubated with secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 546, Thermo Fisher Scientific, A11034, A11003) at RT for 30 min and washed with PBS. .. After sample preparation, the smart kit imaging buffer (Abbelight) was added to the cells prior to SMLM imaging.
Article Title: Super‐resolution microscopy reveals focal organization of ER ‐associated Y‐complexes in mitosis
Article Snippet: .. In brief, Nup96‐SNAP was labeled with the BG‐AF647 dye (New England Biolabs, S9136S) at a concentration of 1 μM in 0.5% BSA in PBS at RT for 2 h. The cells were then incubated with primary antibodies (phospho‐H3, Climp63) for 1 h. Subsequently, cells were washed with PBS and then incubated with secondary antibodies (goat anti‐rabbit Alexa Fluor 488, goat anti‐mouse Alexa Fluor 546, Thermo Fisher Scientific, A11034, A11003) at RT for 30 min and washed with PBS. .. After sample preparation, the smart kit imaging buffer (Abbelight) was added to the cells prior to SMLM imaging.
Incubation:Article Title: Super-resolution microscopy reveals focal organization of ER-associated Y-complexes in mitosis.
Article Snippet: .. In brief, Nup96-SNAP was labeled with the BG-AF647 dye (New England Biolabs, S9136S) 10 of 13 EMBO reports 24: e56766 | 2023 2023 The Authors D ow nloaded from https://w w w .em bopress.org on January 25, 2024 from IP 220.158.144.59. at a concentration of 1 lM in 0.5% BSA in PBS at RT for 2 h. The cells were then incubated with primary antibodies (phospho-H3, Climp63) for 1 h. Subsequently, cells were washed with PBS and then incubated with secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 546, Thermo Fisher Scientific, A11034, A11003) at RT for 30 min and washed with PBS. .. After sample preparation, the smart kit imaging buffer (Abbelight) was added to the cells prior to SMLM imaging.
Article Title: Super‐resolution microscopy reveals focal organization of ER ‐associated Y‐complexes in mitosis
Article Snippet: .. In brief, Nup96‐SNAP was labeled with the BG‐AF647 dye (New England Biolabs, S9136S) at a concentration of 1 μM in 0.5% BSA in PBS at RT for 2 h. The cells were then incubated with primary antibodies (phospho‐H3, Climp63) for 1 h. Subsequently, cells were washed with PBS and then incubated with secondary antibodies (goat anti‐rabbit Alexa Fluor 488, goat anti‐mouse Alexa Fluor 546, Thermo Fisher Scientific, A11034, A11003) at RT for 30 min and washed with PBS. .. After sample preparation, the smart kit imaging buffer (Abbelight) was added to the cells prior to SMLM imaging.
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